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ECM830电穿孔仪
ECM830电穿孔仪
订货号: MM-58125-00
产地: 美国
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开发编号: Y
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Applications

Cell biology research frequently needs the ability to transfect hundreds if not thousands of samples at once. Using traditional electroporation techniques, such as cuvettes, would be time-consuming and expensive, if not impossible. High throughput electroporation allows the user to accelerate research by conducting multiple experiments at one time on a single plate.
 
Advanced Transfection and Transformation
When a large number of samples need to be transfected, minimizing the number of separate transfer steps saves time and work and therefore ultimately money. In the quest for more efficient methods, assay systems are being miniaturized to allow the handling of more samples in 96 or 25 well formats.
High throughput electroporation applications in transfection include genomic library construction, delivery of siRNA, genome sequencing studies, subcloning, mutagenesis, and directed evolution studies.
 
High Throughput Screening for Target ID and Validation
Biomolecular screening is one of the principal tools used for the discovery of new drugs in the biopharmaceutical industry today. Batches of compounds are tested for binding activity or biological activity against target molecules. High throughput screening seeks to screen large numbers of compounds rapidly and in parallel.
 
Electroporation transfection applications in this context include antisense oligonucleotide delivery and siRNA delivery to cells for identification and validation of potential targets.
 
Genomics and Proteomics
Transfection is a valuable way to investigate gene function in cells. Recent advances in genomics, the study of genes and their function, are bringing about a revolution in our understanding of the molecular mechanisms of disease, including the complex interplay of genetic and environmental factors.
 
Genomics is also stimulating the discovery of breakthrough healthcare products by revealing thousands of new biological targets for the development of drugs, and by giving scientists innovative ways to design new drugs, vaccines, and DNA diagnostics.
 
Sequencing the genome established a firm foundation-but proteomics, the study of the full set of proteins encoded by a genome, is the next, more challenging phase in the process of understanding cellular biochemistry and mechanisms of disease. High throughput electroporation will facilitate this exciting area of research.
 
Technical Specifications
Standard Capabilities
 
     
Interface:
     
Digital User Interface
 
 
Input:
 
110 V/220 V Universal
 
 
Charge Time:
 
13 sec maximum
 
 
Voltage Range:
 
5 - 500 V LV Mode/ 1 V resolution
505 - 3000 V HV Mode/ 5 V resolution
 
 
Pulse Length Range:
 
1 ms - 999 ms LV Mode/ 1 ms resolution
10 µsec - 999µsec LV Mode/ 1 µsec resolution
1 sec - 10 sec LV Mode/ 0.1 sec resolution
10 µs - 600 µs HV Mode/ 1 µs resolution
 
 
Multiple Pulsing:
 
1 - 99 pulses
 
 
Pulse Interval:
 
100 msec - 10 sec
 
 
Programmability:
 
Storage for 3 setups (V, t, n, interval)
 
 
Arc Control:
 
Arc Quenching
 
 
 
Other Electrical Characteristics
 
 
Capacitance:
 
4000 µF LV, 110 µF HV
 
 
Amperage:
 
500 A LV, 100 A HV
 
 
 
Physical Characteristics
 
 
Footprint:
 
12.5" x 12.25" x 5.5" (14 x 31.8 x 31.1 cm) (W x D x H)
 
 
Weight:
 
15 lbs. (6.8 kg)
 
 
Display:
 
20 x 4 character LCD

 
 
Controls:
 
Single Rotary Encoder with push button toggle between all set parameters.
Additional on/off Power and Start switches
 
 
 
Monitoring:
 
Displays all set parameters after pulsing
 
 


品牌简介

美国著名BTX是专业的细胞融合、电穿孔仪的生产厂家。自从1983年起,苛求的科研工作者就已经把BTX产品作为电融合、转基因等应用领域的首选仪器。除了占世界领导地位的电融合、…美国著名BTX是专业的细胞融合、电穿孔仪的生产厂家。自从1983年起,苛求的科研工作者就已经把BTX产品作为电融合、转基因等应用领域的首选仪器。除了占世界领导地位的电融合、转基因系统外,BTX的产品还包括多达25种相关的配件,其中有多种专业的电极,给用户更多的选择。BTX还在网站建立了技术数据库,提供超过5000份的参考目录和600多份的protocols供科研工作者免费查阅。ECM系列产品在转基因的应用领域中得以广泛的应用,其无以伦比的高性能得到了全球同行的厚爱。BTX的系列产品适用于动植物、细菌、酵母、哺乳动物、人类细胞(包括活体细胞)的转基因等操作。

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