Gradient Thermal Cyclers

Techne now offer two gradient units; the new TC-5000 full-sized and the TC-3000G personal thermal cyclers

TC-5000, 96 or 384-well

• Versatile block format: User-friendly, fully interchangeable block system allows blocks to be exchanged in a matter of seconds without the need for any tools (60 x 0.5ml, 96 x 0.2ml, 96-well fully skirted plates, 384-well or flat plate for In-situ).
• Defined pressure, programmable heated lid (100°C to 115°C) to accommodate a variety of consumables. The heated lid only comes on if the block is set above 35°C.
• Temperature range of 4°C to 99°C
• 8 peltiers: Each block consists of 8 Peltier units, controlled by 4 independent temperature thermistors (control sensors) distributed evenly across the block
•Incremental/decremental time and temperature for applications such as Touchdown PCR
• The TC-5000 can be connected to a PC along with any other Techne cyclers for high throughput applications using Gensoft software
• "Real-time" graphical display shows the sample temperature profile while the program is running, including the upper and lower limits of the gradient. This pictorial, real-time representation means instant visualisation of your experiment?s status.
•User-friendly programming interface provided by a 115 x 90mm touch-sensitive screen.
•Excellent heating rate: A maximum heating rate of 3°C/sec and block uniformity of ±0.3°C at 50°C with or without a gradient...no compromise!
•The most linear gradient cycler on the market, with an amazing range of 30°C.
•The gradient calculator function displays the temperature of each column of tubes. This ensures replication of the experimental conditions.
•Gradient range 20°C to 70°C, maximum 30°C
•Decremental/incremental time and temperature

TC-3000G, 48-well

The world's smallest gradient cycler, the TC-3000G will hold half a 96-well plate, in a horizontal format so that it offers 8 columns for annealing temperature optimisation and 6 rows for optimising reagents such as MgCl2 and primer concentrations. Annealing temperatures can be optimised over a 15°C gradient between the temperatures of 20 and 80°C.

• Adjustable heated lid: The heated lid can now be adjusted for use with all PCR tubes, strip tubes or 48-well plates sealed with sealing mats
• Rapid heating rate: Utilising the latest Peltier technology the TC-3000G has a heating ramp rate of 3.3°C/sec and a 2.0°C/sec cooling rate
• Fast-track programming: Modern intuitive programming and ready-to-go templates means creating even the most complicated protocols is simple
• PC control & networking: The TC-3000X can be linked together with other Techne cyclers; up to 32 cyclers can be networked to one computer
• Gradient block: A gradient of up to 15°C between the temperatures of 20 and 80°C can be set, allowing any protocol to be optimised within a single experiement.
• New software: Enables a gradient to be added to any temperature step of the program.
• Gradient calculator: The gradient calculator function displays the temperature for each of the 8 columns, ensuring easy replication of thermal conditions.

Technical Specification

 

Parameters	TC-3000G	TC-5000
0.5ml block capacity	30	60
0.2ml block capacity	48	96
384-well block	NA	Yes
In-situ block	NA	Yes
Temperature range	4°C to 99°C	4°C to 99°C
Maximum ramp rate	3.3°C/sec	3.0°C/sec
Gradient range	20°C to 80°C	20°C to 70°C
Maximum gradient	15°C	30°C (16°C for 384 block)
Block uniformity at 50°C	±0.2°C	<±0.3°C
Peltiers per block	6	8
Selectable heatd lid temperature	100°C to 115°C	100°C - 115°C
Heated lid enable/disable	Yes	Yes
No. of programs	80	50
Programming display	4-line alphanumeric	Touchscreen
Graphical display	NA	Real-Time Graph
Auto restart on power failure	Yes	Yes
Dimensions L x W x H	330 x 170 x 190mm	420 x 220 x 260mm
Voltage	230/115V/100V 50-60Hz	Dual voltage 230/115V 50-60Hz
Power	230W	620W
Shipping weight	6kg	14kg