ECM399电穿孔仪

添加时间：2010-1-16 编辑：C055 阅读：次

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产品名称：ECM399电穿孔仪
产品定货号：ML-07525-00

产地：美国
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Description

The ECM 399 is an electroporation system specifically designed to produce the precise field strengths and pulse lengths required for the transfection of mammalian and transformation of bacterial and yeast cells. The ECM 399 utilizes the new BTX Power Platform Technology design and novel digital user interface. The single-dial encoder simplifies the operation of ECM 399. Portable, convenient, and user-friendly are major features of this unit.

Applications

Bacteria/Yeast Transformation
In the High Voltage (HV) Mode, the ECM 399 is optimized to electroporate E. coli and other bacteria and yeast for construction of cDNA libraries or mutagenesis.

Mammalian Cell Transfection
In the Low Voltage (LV) Mode, the ECM 399 is designed to deliver a pulse length of approximately 26 msec using the 4mm gap cuvette, at a volume of 400µl using conductive media such as PBS. This protocol has been found to be ideal for transfection of lymphoid, myeloid, glial, fibroblast and Cos 7 cells.1 Protocols for these and other mammalian cells can be optimized by varying the voltage and/or the pulse length. Adjusting the voltage adjustment knob varies the voltage, while the pulse length may be changed by varying the sample volume or buffer composition.

Technical Specifications

Standard Capabilities

Operational Status:		Internal Self Test Upon Start-up
	Interface:	Digital User Interface
	Charge Time:	5 sec maximum
	Voltage Range:	2-500 V LV Mode/ 2 V resolution 10-2,500 V HV Mode/10 V resolution
	Capacitance:	1050 µF LV Mode 36 µF in HV Mode
	Resistance:	150 ohms both LV and HV Modes
	Safety:	Short circuit and arc proof

Physical Characteristics
	Footprint:	9.1"x7.7"x4.3"(WxDxH)
	Weight:	7 pounds
	Display:	16-character LCD backlit
	Controls:	Single rotary encoder for voltage selection. Additional on/off power and start switches

Monitoring		Monitoring and display of V p and RC time constant