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添加时间:2009-8-7 编辑:泰亚赛福 阅读:次
DNA Engine Opticon 2 双通道实时定量PCR 仪
【介绍】
DNA Engine Option 2 是一款双波长实时PCR 仪。96 个蓝-绿 LED 顺序激发样品,通过二个光电二极管来检测。两个激发波长范围: 一为523–543nm(适用于FAM、SYBR Green I 等),还有一个是 540–700nm(适用于普遍使用的HEX、TEMRA、TET、VIC 等荧光素)。
DNA Engine Option 2 既可以支持普通的单重PCR,也可以支持多重 PCR。多重 PCR 除了可以节省试剂和时间外,还可以使内对照的使用成为可能,从而对样品PCR 效率有更进一步的掌控。此外,多重PCR 也 使基因表达分析更为简便。其具体性能如下:
一次96 孔荧光检测时间在3 秒之内,最大程度减少了荧光交叉
完美的温度控制性能和梯度PCR 功能
Opticon Monitor 分析软件可以非常简单地设置RT–PCR、多重PCR 等反应,以及DNA 定量、基因表达、等位基因等功能分析
LED 阵列与反应板一一对应,保证激发光准确进入目标管。发射光通过光电倍增管(PMTs)系统进行信号放大。
【技术指标】
Base thermal cycler |
DNA Engine |
Sample capacity |
96-well PCR plate (low-profile) or 12 x 0.2 ml 8-tube strips (low-profile) |
Sample volume |
10–100 µl (20 µl recommended) |
Dimensions (W x D x H) |
34 x 47 x 60 cm |
Weight |
29 kg |
Fluorescence excitation range |
470–505 nm |
Fluorescence detection range |
Channel 1: 523–543 nm |
Linear dynamic range of starting copy number |
Up to 10 orders of magnitude |
Detection limit of template starting copy number |
Down to single initial template copy detection |
Gradient Specifications | |
Gradient range |
30–105°C |
Temperature differential range |
1–24°C |
Gradient accuracy |
±0.3°C of target at end columns within 30 sec of arrival at programmed target, NIST–traceable |
Temperature uniformity |
±0.4°C, in column, well-to-well, within 10 sec of target attainment |
Gradient calculator accuracy |
±0.4°C of actual column temperature (NIST–traceable) |
Computer System Requirements (Computer NOT Included With System) | |
Model |
Desktop tower computer |
PCI card slot dimensions |
Full-size card, 280 mm (11 in) x 106 mm (4.2 in) or short card, 174 mm (6.9 in) x 106 mm (4.2 in) |
Operating system |
Windows XP Professional |
Storage capacity |
40 GB hard drive |
Memory |
512 MB RAM |
Port |
Communication (COM) |
Supplied With System | |
Data acquisition board |
National Instruments PCI-6036E, 200 x 10³ samples/sec |
Note: Specifications subject to change without notice. |
产品名称 | 产品简介 | 产品编号 |
---|---|---|
DNA Engine Opticon2双通道实时定量PCR仪 | ML-03004-00 |