Base Thermal Cycler |
DNA Engine or Dyad Disciple cycler |
Sample capacity |
96-well PCR plate or 96 x 0.2 ml tubes |
Sample volume |
10–100 µl (20 µl recommended) |
Dimensions (W x D x H) |
23 x 23 x 18 cm (excluding thermal cycler) |
Weight |
6.8 kg (excluding thermal cycler) |
Fluorescence excitation range |
Channel 1: 450–490 nm Channel 2: 500–535 nm Channel 3: 555–585 nm Channel 4: 620–650 nm |
Fluorescence detection range |
Channel 1: 515–530 nm Channel 2: 560–580 nm Channel 3: 610–650 nm Channel 4: 675–730 nm |
Linear dynamic range of starting copy number |
Up to 10 orders of magnitude |
Detection limit of template starting copy number |
Down to single initial template copy detection |
Gradient specifications |
Gradient range |
30–105°C |
Temperature differential range |
1°C–24°C |
Gradient accuracy |
±0.3°C of programmed target at end columns within 30 sec after gradient step timer starts, NIST traceable |
Temperature uniformity |
±0.4°C, in column, well-to-well, within 30 sec of reaching target temperature |
Gradient calculator accuracy |
±0.4°C of actual well temperature |
Note: Specifications subject to change without notice. |
Minimum Computer Specifications |
Operating system |
Windows XP Professional |
Processor speed |
1.5 GHz |
Storage capacity |
20 GB hard drive |
Communications port |
USB 2.0 high-speed |
Display |
1,024 x 768 pixel minimum screen resolution, 256 colors |
Memory |
512 MB RAM |
Note: Specifications subject to change without notice. |